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Flow Cytometry Calibration Particles

The SPHERO™ Calibration Particles are designed for routine calibration of flow cytometers. By using these particles, the sensitivity and linearity of the system can be determined. The sensitivity of the instrument will help detect any dye or light contamination. The linearity of the instrument will determine if the logarithmic amps and PMTs are functioning properly.

SPHERO™ Rainbow Calibration Particles
- Contains a mixture of fluorophores that enable the Rainbow Calibration Particles to be excited at any wavelength from 365 nm to 650 nm
- Allows the calibration of the FITC, PE, PE-TR, PE-Cy5, and APC channels in the flow cytometer with the same set of particles
- Consists of set of several similar size particles with different fluorescence intensities in a convenient dropper bottle
- Can be stored in the freezer and reused later since they can withstand repeated freezing and thawing
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
RCP-20-5
Rainbow Calibration Particles, 4 peaks
107/mL
1.8-2.2 µm
5 mL
$230.00
RCP-30-5 (6 peaks)
Rainbow Calibration Particles, 6 peaks
107/mL
3.0-3.4 µm
5 mL
$250.00
RCP-30-5A (8 peaks)
Rainbow Calibration Particles, 8 peaks
107/mL
3.0-3.4 µm
5 mL
$250.00
RCP-32-5
Rainbow Calibration Particles, 6 peaks
107/mL
3.2 (+/- 0.1) µm
5 mL
$250.00
RCP-35-5
Rainbow Calibration Particles, 4 peaks
107/mL
3.5-4.0 µm
5 mL
$250.00
RCP-60-5
Rainbow Calibration Particles, 6 peaks
107/mL
6.0-6.4 µm
5 mL
$250.00
RCP-30-5A-2
Rainbow Calibration Particles, 2nd peak from the 8 peak set
107/mL
3.0-3.4 µm
5 mL
$250.00
RCP-30-20A
Rainbow Calibration Particles, 8 peaks
107/mL
3.0-3.4 µm
20 mL
$800.00
Downloadable NOTE Rainbow Calibration Particles
ADDITIONAL NOTES:
- Other Rainbow Calibration Particles individual peaks are available upon request. Please contact customer service.
- To see how our customers are using the RCP-30-5A for System Performance Verification of the Beckman Coulter CytoFLEX

SPHERO™ Rainbow Calibration Particles (Euroflow)
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
RCP-30-5A (Euroflow)
Rainbow Calibration Particles, 8 peaks, Lot EAF01
107/mL
3.0-3.4 µm
5 mL
$250.00

Downloadable Technical Data Sheets with Target values for RCP-30-5A (Euroflow):
RCP-30-5A(Euroflow) Lot# EAD05 RCP-30-5A(Euroflow) Lot# EAE01 RCP-30-5A(Euroflow) Lot# EAF01
ADDITIONAL NOTES:
- Catalog No. RCP-30-5A (EUROFLOW) Lot No. EAF01 is the current lot reserved for the Euroflow Group. Please indicate this lot number when placing an order. Target values for Catalog No. RCP-30-5A (EUROFLOW) Lot No. EAF01 are listed below:
SPHERO™ Ultra Rainbow Calibration Particles
- Designed for routine calibration of the flow cytometer in all channels
- Contain a mixture of fluorophores that enable the optimization of linearity, sensitivity, and resolution of the flow cytometer
- Especially useful in the Far Red and Violet channels
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
URCP-38-2K
Ultra Rainbow Calibration Kit, 6 peaks
(Blank, 2x106/mL), (Fluorescent Particles, 107/mL)
107/mL after mixing
3.5-3.9 µm
1x3 mL
1x2 mL
$240.00
URCP-38-20K
Ultra Rainbow Calibration Kit, 6 peaks
(Blank, 2x106/mL), (Fluorescent Particles, 107/mL)
107/mL after mixing
3.5-3.9 µm
1x30 mL
1x20 mL
$1920.00
URCP-50-2K
Ultra Rainbow Calibration Kit, 6 peaks
(Blank 2x106/mL), (Fluorescent Particles 107/mL)
107/mL after mixing
5.0-5.4 µm
1x3 mL
1x2 mL
$245.00
URCP01-30-10K
Ultra Rainbow Calibration Particle Kit, 3 peaks
106/mL
3.0-3.4 µm
10x3 mL
$360.00
URCP-100-2
Ultra Rainbow Calibration Particles, 6 peaks
107/mL
8.0-12.9 µm
2 mL
$245.00

SPHERO™ Spectral Matching Calibration Particle Kits
- Surface labeled polystyrene particles with commonly used fluorophores
- Used to determine the linearity of the logarithmic amps and sensitivity of flow cytometers in specific channels
- Supplied as individual products for use in specific channels of flow cytometers
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
ECFP-F1-5K
FITC Easy Calibration Kit, 5 peaks
2x106/mL
3.0-3.4 µm
5x1 mL
$245.00
ECFP-F2-5K
PE Easy Calibration Kit, 5 peaks
2x106/mL
3.0-3.4 µm
5x1 mL
$245.00
ECFP-F4-5K
PE-Cy5 Easy Calibration Kit, 5 peaks
2x106/mL
3.0-3.4 µm
5x1 mL
$245.00
ECFP-F9-6K
GFP Easy Calibration Kit, 6 peaks
2x106/mL
3.0-3.4 µm
6x1 mL
$250.00

SPHERO™ Rainbow Linear Calibration Particles
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
RLP-30-5
Rainbow Linear Calibration Particles (For use in Linear Scale)
107/mL
3.0-3.4 µm
5 mL
$250.00

SPHERO™ APC Calibration Particles
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
ACP-30-5K
APC Calibration Particle Kit (6 peaks)
107/mL
3.0-3.4 µm
2x5 mL
$240.00

SPHERO™ IR Calibration Particles
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
FCP-3580-2
Fluorescent Calibration Particles, 800nm EM, 4 peaks
107/mL
3.5 µm (+/- 0.2 µm)
2 mL
$230.00

SPHERO™ Yellow Calibration Particles
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
YCP-70-5
Yellow Calibration Particles
107/mL
6.0-8.0 µm
5 mL
$260.00