FLOW CYTOMETRY CALIBRATION PARTICLES

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Flow Cytometry Calibration Particles

The SPHERO™ Calibration Particles are designed for routine calibration of flow cytometers. By using these particles, the sensitivity and linearity of the system can be determined. The sensitivity of the instrument will help detect any dye or light contamination. The linearity of the instrument will determine if the logarithmic amps and PMTs are functioning properly.

SPHERO™ Rainbow Calibration Particles - Contains a mixture of fluorophores that enable the Rainbow Calibration Particles to be excited at any wavelength from 365 nm to 650 nm - Allows the calibration of the FITC, PE, PE-TR, PE-Cy5, and APC channels in the flow cytometer with the same set of particles - Consists of set of several similar size particles with different fluorescence intensities in a convenient dropper bottle - Can be stored in the freezer and reused later since they can withstand repeated freezing and thawing
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
RCP-20-5	Rainbow Calibration Particles, 4 peaks	10⁷/mL	0.4-0.6 µm	5 mL	$260.00
RCP-20-5	Rainbow Calibration Particles, 4 peaks	10⁷/mL	1.8-2.2 µm	5 mL	$245.00
RCP-30-5 (6 peaks)	Rainbow Calibration Particles, 6 peaks	10⁷/mL	3.0-3.4 µm	5 mL	$265.00
RCP-30-5A (8 peaks)	Rainbow Calibration Particles, 8 peaks	10⁷/mL	3.0-3.4 µm	5 mL	$265.00
RCP-30-20A	Rainbow Calibration Particles, 8 peaks	10⁷/mL	3.0-3.4 µm	20 mL	$850.00
RCP-30-5A-2	Rainbow Calibration Particles, 2nd peak from the 8 peak set	10⁷/mL	3.0-3.4 µm	5 mL	$265.00
RCP-32-5	Rainbow Calibration Particles, 6 peaks	10⁷/mL	3.2 (+/- 0.1) µm	5 mL	$265.00
RCP-35-5	Rainbow Calibration Particles, 4 peaks	10⁷/mL	3.5-4.0 µm	5 mL	$265.00
RCP-60-5	Rainbow Calibration Particles, 6 peaks	10⁷/mL	6.0-6.4 µm	5 mL	$265.00
RCP-60-20	Rainbow Calibration Particles, 6 peaks	10⁷/mL	6.0-6.4 µm	20 mL	$850.00
RQC-30-5	Rainbow QC Calibration Particles, 3 peaks	2x10⁷/mL	3.0-3.4 µm	5 mL	$490.00

Downloadable NOTE Rainbow Calibration Particles
ADDITIONAL NOTES:
- Other Rainbow Calibration Particles individual peaks are available upon request. Please contact customer service.
- To see how our customers are using the RCP-30-5A for System Performance Verification of the Beckman Coulter CytoFLEX
- STN-8: Calibration and Performance Tracking of Flow Cytometer Using SPHERO Calibration Particles
- STN-9: Measuring MEF with Flow Cytometer Using SPHERO™ Rainbow Calibration Particles
- STN-14: Determining PMT Linearity in Flow Cytometers Using the SPHERO PMT Quality Control Excel Template
- STN-17: Determination of a Flow Cytomerter's Sensitivity Using Detection Efficiency (Q) and the Background Light Level (B)
- PMT QC Templates can be downloaded at https://www.spherotech.com/templates.html

SPHERO™ Rainbow Calibration Particles (Euroflow)
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
RCP-30-5A (Euroflow)	Rainbow Calibration Particles, 8 peaks, Lot EAL01	10⁷/mL	3.0-3.4 µm	5 mL	$265.00

Downloadable Technical Data Sheets with Target values for RCP-30-5A (Euroflow):
RCP-30-5A(Euroflow) Lot# EAK01, EAG01, EAE01 & EAL01
RCP-30-5A(Euroflow) Lot# EAM01

ADDITIONAL NOTES:
- Catalog No. RCP-30-5A (EUROFLOW) Lot No. EAM01 is the current lot reserved for the Euroflow Group. Please indicate this lot number when placing an order.
- Downloadable Product Details: EuroFlow Rainbow Calibration Particles
- Access to the EuroFlow SOPs can be found at https://euroflow.org/usr/pub/pub.php

Target values for Catalog No. RCP-30-5A (EUROFLOW) Lot No. EAM01 are listed below:

SPHERO™ Ultra Rainbow Calibration Particles - Designed for routine calibration of the flow cytometer in all channels - Contain a mixture of fluorophores that enable the optimization of linearity, sensitivity, and resolution of the flow cytometer - Especially useful in the Far Red and Violet channels
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
URCP-38-2K	Ultra Rainbow Calibration Kit, 6 peaks (Blank, 2x10⁶/mL), (Fluorescent Particles, 10⁷/mL)	10⁷/mL after mixing	3.8 µm (+/- 0.2 µm)	1x3 mL 1x2 mL	$255.00
URCP-38-20K	Ultra Rainbow Calibration Kit, 6 peaks (Blank, 2x10⁶/mL), (Fluorescent Particles, 10⁷/mL)	10⁷/mL after mixing	3.8 µm (+/- 0.2 µm)	1x30 mL 1x20 mL	$2040.00
URCP-50-2K	Ultra Rainbow Calibration Kit, 6 peaks (Blank 2x10⁶/mL), (Fluorescent Particles 10⁷/mL)	10⁷/mL after mixing	5.0-5.4 µm	1x3 mL 1x2 mL	$260.00
URCP01-30-10K	Ultra Rainbow Calibration Particle Kit, 3 peaks	10⁶/mL	3.0-3.4 µm	10x3 mL	$375.00
URCP-100-2	Ultra Rainbow Calibration Particles, 6 peaks	10⁷/mL	8.0-12.9 µm	2 mL	$260.00
URCP-100-2H	Ultra Rainbow Calibration Particles, 9 peaks	10⁷/mL	8.0-12.9 µm	2 mL	$305.00
URCP-100-6K	Ultra Rainbow Calibration Kit, 6 peaks	10⁷/mL after mixing	8.0-12.9 µm	6x2 mL	$1040.00

Downloadable NOTE Ultra Rainbow Calibration Particles
ADDITIONAL NOTES:
- STN-8: Calibration and Performance Tracking of Flow Cytometer Using SPHERO Calibration Particles
- STN-9: Measuring MEF with Flow Cytometer Using SPHERO™ Rainbow Calibration Particles
- STN-14: Determining PMT Linearity in Flow Cytometers Using the SPHERO PMT Quality Control Excel Template
- STN-17: Determination of a Flow Cytomerter's Sensitivity Using Detection Efficiency (Q) and the Background Light Level (B)
- PMT QC Templates can be downloaded at https://www.spherotech.com/templates.html

SPHERO™ Spectral Matching Calibration Particle Kits - Surface labeled polystyrene particles with commonly used fluorophores - Used to determine the linearity of the logarithmic amps and sensitivity of flow cytometers in specific channels - Supplied as individual products for use in specific channels of flow cytometers
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
ECFP-F1-5K	FITC Easy Calibration Kit, 5 peaks	2x10⁶/mL	3.0-3.4 µm	5x1 mL	$260.00
ECFP-F2-5K	PE Easy Calibration Kit, 5 peaks	2x10⁶/mL	3.0-3.4 µm	5x1 mL	$260.00
ECFP-F4-5K	PE-Cy5 Easy Calibration Kit, 5 peaks	2x10⁶/mL	3.0-3.4 µm	5x1 mL	$260.00
ECFP-F9-6K	GFP Easy Calibration Kit, 6 peaks	2x10⁶/mL	3.0-3.4 µm	6x1 mL	$265.00

Downloadable NOTE Spectral Matching Calibration Particle Kits
ADDITIONAL NOTES:
- STN-9: Measuring MEF with Flow Cytometer Using SPHERO™ Rainbow Calibration Particles

SPHERO™ Rainbow Linear Calibration Particles
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
RLP-30-5	Rainbow Linear Calibration Particles (For use in Linear Scale)	10⁷/mL	3.0-3.4 µm	5 mL	$265.00

SPHERO™ APC Calibration Particles
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
ACP-30-5K	APC Calibration Particle Kit (6 peaks)	10⁷/mL	3.0-3.4 µm	2x5 mL	$255.00

SPHERO™ IR Calibration Particles
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
FCP-34800-6K	Fluorescent Calibration Particles, 800nm EM, 6 peaks	10⁷/mL	3.5 µm (+/- 0.2 µm)	6x1 mL	$260.00
FCP-3580-2	Fluorescent Calibration Particles, 800nm EM, 4 peaks	10⁷/mL	3.5 µm (+/- 0.2 µm)	2 mL	$245.00

SPHERO™ Yellow Calibration Particles
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
FCP-7052-5	Fluorescent Calibration Particles, Yellow (6 peaks)	10⁷/mL	7.0-7.9 µm	5 mL	$250.00
YCP-70-5	Yellow Calibration Particles (5 peaks)	10⁷/mL	6.0-8.0 µm	5 mL	$275.00

Downloadable NOTE Various Calibration Particles Products