Flow Cytometry

To ensure accuracy and consistency in flow cytometry assays, Spherotech offers flow cytometry beads.

• Flow cytometry alignment, calibration, compensation, and enumeration
• Cell sorter drop delay calibration
• Signaling analysis
• Stem cell analysis
• Immunophenotyping
• Quantitative flow cytometry

Cell Labeling and Tracking

To allow for the detailed analysis of cells using fluorescent tagging, Spherotech offers fluorescent nanobeads.

• Enables flow cytometry, microscopy, and fluorescence-activated cell sorting (FACS)
• Available in multiple sizes and fluorescence wavelengths
• Immunostaining and Cell Identification and Separation
• Streptavadin coated flourescent microbeads capture biotinylated antibodies, ligands, and other biomolecules
• Often employed in sandwich assays

Cell Separation

When you need to facilitate cell separation from heterogenous samples, Spherotech recommends magnetic beads.

• Can be functionalized with specific antibodies or peptides that bind to target cells
• Coated with specific antibodies to bind to cell surface markers selectively

Multiplex Bead Arrays

Particle Array Kits (PAKs) are effective for research situations where multiple cell targets must be identified and analyzed simultaneously.

• Multiplexing assay kits with carboxyl functionalized microbeads
• Varying fluorescence intensities available

Magnetic Separation

To aid in isolating cell populations from mixed samples, Spherotech offers magnetic separation beads.

• Can be coated with specific antibodies or ligands to bind to specific cell types
• Used in applications such as separating leukocyte subsets from whole-blood

Fluorescence-Based Sorting

When cells need to be identified and sorted based on fluorescence, Spherotech recommends ligand-coated fluorescent magnetic nanobeads.

• Can be used in flow cytometry and fluorescence-activated cell sorting (FACS) systems
• Used to separate cells tagged with fluorescent markers

Biotin-based Cell Binding

For cell separation tasks in which you need custom beads formed by binding biotinylated antibodies or biotin-tagged molecules, Spherotech recommends streptavidin coated magnetic microbeads.

• Facilitates cell isolation based on unique surface markers or proteins

Protein Analysis

To aid in the detection of target proteins in biological samples, Spherotech offers functionalized polystyrene beads.

• Can be coated with proteins and ligands
• Often used to make bead-based assays for protein quantification, protein-protein interaction studies, and protein purification

Flow Cytometry Controls

For optimizing and standardizing flow cytometers for cellular, genomic, and proteomic research, Spherotech recommends flow cytometry calibration, alignment and compensation particles.

• Ensure accuracy and reproducibility of flow cytometry data
• Allows for reliable quantitative analysis of biomolecules like proteins or nucleic acids

Protein Quantification, Sorting, and Molecular Imaging

Spherotech’s ligand and protein-coated fluorescent microspheres and nanospheres are commonly used for targeted specific analyte isolation and detection, or in the development of customized multiplex assays for multiple analyte detection simultaneously.

• Available in various sizes and fluorophores

Affinity Purification

To aid in affinity purification techniques where proteins or protein complexes must be isolated from biological samples, Spherotech offers streptavidin coated magnetic and polymer microspheres.

• Offer high-affinity binding to biotin-tagged molecules
• Promotes efficient purification for downstream genomics and proteomics analysis

Nucleic Acid Purification

For efficient isolation and purification of DNA or RNA from biological samples, Spherotech recommends magnetic and polymer OdT, antibody, and protein coated microspheres.

• Functionalized with nucleic acid-binding molecules like oligonucleotides or common protein conjugates ready to scaffold sequences
• Aids in downstream molecular biology workflow and analyses like PCR, sequencing, or cloning

Multiple Analyte Detection and Quantification

With Spherotech’s particle array kits, you can develop multiplex assays to detect and quantify multiple analytes in a single sample.

• Include microspheres coated with different capture molecules
• Facilitates efficient and cost-effective molecular analysis

Molecular Probing

To aid in molecular biology applications involving biotinylated probes, Spherotech recommends its streptavidin coated microspheres.

• Often used in nucleic acid hybridization assays or protein pull-down experiments
• Versatile platform for immobilizing biotinylated molecules with high affinity and specificity

Antigen/Antibody Immobilization

Spherotech recommends high surface area Fluorescently Labeled Microspheres to serve as the solid phase in immunoassays.

• Available in a variety of emission spectra
• Allow for detection of multiple analytes in a single assay

Biotinylated Antibody Detection

To enable the capture and detection of biotinylated antibodies or antigens, Spherotech offers Streptavidin Coated Microspheres.

• Provide a strong and stable biotin-streptavidin interaction
• Enhance assay sensitivity and specificity

Flow Cytometry Control

To aid in optimizing and standardizing flow cytometry-based immunoassays, Spherotech suggests Calibration Beads and Compensation Beads.

• Help to validate instrument performance
• Ensure accurate quantification of target analytes

Protein and Ligand-based Immunoassays

In assay applications in which coatings are required for proteins and ligands, Spherotech recommends Functionalized Polystyrene Beads.

• Ideal for enzyme-linked immunosorbent assays (ELISA)
• Also effective in immunofluorescence assays and bead-based assays